Trypsin and α-amylase are the main digestive enzymes in the gut
and hepatopancreas of shrimp and play a key role in the assimilation
of nutrition (Muhlia-Almazán et al., 2003). Until now, most studies
have investigated the effects of diets with different carbohydrate concentrations,
biofloc, protein, or probiotics on trypsin and α-amylase
(Arena et al., 2007; Anand et al., 2014; Ziaei-Nejad et al., 2006), but
few studies have reported on their expression levels. In this study, the
mRNA transcripts of trypsin and α-amylase were investigated when
shrimp were fed with treatment diets. We found that E. faecium NRW-
2 had a stronger induction of these two digestive enzymes in the midgut
at all time points, while the L. pentosus HC-2 and their supernatant contributed
to the expression of digestive enzymes in the hepatopancreas