Gluconacetobacter xylinus from the
agar plate was inoculated in Schramm
Hestrin medium (SH) placed in a conical
flask. The culture medium was
previously sterilised at 121 °C for
20 minutes. After the inoculation process,
which took 2 days and was performed in
an incubator at 30 ± 2 °C, the culture medium
with bacteria in an amount of about
5% volume was transferred to the next
culture medium.