Cellular oxidative damage treatment. NHF cells were damaged by
H2O2 (Merck) due to its pivotal role in generating reactive oxygen
species from nearly all sources of oxidative stress, and oxygen radicals
can diffuse freely in and out of cells and tissues.6,20) NHF cells
(8 103 cells/well) were treated as above. Cytotoxicity was induced
by H2O2 (1 103{1 103 mg/mL) for 24 h. The untreated cells were
used as control. The treatment of cells, and determination of cell
viability was conducted as above. The concentration of H2O2 causing
cell damaged was obtained. Thus that concentration was applied to
treat NHF cells (8 103 cells/well) for 24 h, and the cells were treated
with the samples for a further 24 h. Determination of cell viability was
conducted as above.18,21)