Enzymatic digestion of amplified DNA
The digestion mixture was incubated for 16h. The incubatino termparature was chosen as recromme by the manufacton of optimal result the digested samples were analyzed by electrophoresis by using 3% agarose gel in 1 x TBE Buffer, ph 8.0 for 1 h at 90 V and stained by ethidium bromide. A 100 bp DNA ladder was used as size reference.