In addition to the research of stabilization of RBO by sub-CW
treatment, extraction of RBO was also studied. Conventional rapid
solid–liquid extractions of RBO were investigated and compared
with sub-CW extraction. The conventional extraction methods
were conducted in three media: hexane–bran, hexane–water–
bran, and ethanol–bran. For hexane–bran, 50.0 g fresh bran was
mixed with 300.0 g hexane and they were shaken for 240 min at
150 rpm and 25 C. At the second direct method, 50.0 g fresh bran,
100.0 g distilled water, and 200.0 g hexane were mixed and shaken
under similar operational conditions to the first option. For rice
bran oil extraction by ethanol, 50.0 g fresh bran was mixed with
300.0 g ethanol and this mixture was shaken for 240 min at
150 rpm and 60 C. The obtained dark green phase was ethanol soluble
(ES) phase which contained oil, and other extractive compounds
by ethanol. After shaking, ES and HS phase were
separated and filtered from other phases and their solvent was
evaporated by rotary evaporator to evaluate the quality of oil,
and also oil extraction yield.