content of pomegranate antioxidants or enhanced their
activity. The FRAP method also showed a higher
antioxidant capacity for the experimental juice produced
from fresh arils with respect to red wine or green tea,
and a smaller activity for the juice produced from frozen
arils (Juice 2). Again, the activity of the commercial
juices (3 and 4) was 2-fold that of the experimental ones
(1 and 2), supporting that the method of juice extraction
has an important role in this activity.
When measuring the antioxidant activity of pomegranate
juices by the DMPD method (Figure 1), an
extraordinary high activity was observed compared to
the other free radical scavenging activity methods.
However, the antioxidant activities of red wine, green
tea, and pomegranate husk extract measured by the
DMPD method were in the same ranges as those
measured with the ABTS, DPPH, and FRAP methods.
This clearly shows that there is something in pomegranate
juice that neutralizes the DMPD free radical,
and that this juice constituent is not a main constituent
in wine, tea, or the water extract of pomegranate husk.
The antioxidant activity for the four analyzed juices
evaluated by the DMPD method was in the same range.
This antioxidant test was repeated at least four times
to confirm the observed high activity. To determine the
reason for the high activity observed in pomegranate
juices with the DMPD method, the commercial single
strength juice (3) was fractionated. A cation-exchange
resin was used to remove anthocyanins from the juice
and the remaining supernatant was fractionated by
filtration trough a C-18 solid-phase extraction cartridge.
content of pomegranate antioxidants or enhanced theiractivity. The FRAP method also showed a higherantioxidant capacity for the experimental juice producedfrom fresh arils with respect to red wine or green tea,and a smaller activity for the juice produced from frozenarils (Juice 2). Again, the activity of the commercialjuices (3 and 4) was 2-fold that of the experimental ones(1 and 2), supporting that the method of juice extractionhas an important role in this activity.When measuring the antioxidant activity of pomegranatejuices by the DMPD method (Figure 1), anextraordinary high activity was observed compared tothe other free radical scavenging activity methods.However, the antioxidant activities of red wine, greentea, and pomegranate husk extract measured by theDMPD method were in the same ranges as thosemeasured with the ABTS, DPPH, and FRAP methods.This clearly shows that there is something in pomegranatejuice that neutralizes the DMPD free radical,and that this juice constituent is not a main constituentin wine, tea, or the water extract of pomegranate husk.The antioxidant activity for the four analyzed juicesevaluated by the DMPD method was in the same range.This antioxidant test was repeated at least four timesto confirm the observed high activity. To determine thereason for the high activity observed in pomegranatejuices with the DMPD method, the commercial singlestrength juice (3) was fractionated. A cation-exchangeresin was used to remove anthocyanins from the juiceand the remaining supernatant was fractionated byfiltration trough a C-18 solid-phase extraction cartridge.
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