Oxidation in meat is usually assessed by measuring the amount of peroxide value (PV),
thiobarbituric acid-reactive substances (TBARS),
sulphydryl and carbonyl group generated during the process.
This analysis is carried out using spectrophotometric or chromatographic
(head space gas chromatographic (GC), high-performance liquid chromatography (HPLC),
liquid chromatographic mass spectrophotometer [(LC–MS) and 2,4 dinitrophenylhy-drazine (DNPH)] methods.
Recently,studies on protein–lipid oxidation have been conducted at a molecular level using mass spectrophotometry (MS) and liquid chromatography–tandem mass spectrophotometer (LC–MS/MS) with proteomic tools to better understand the mode of mechanism in relation to meat quality. Specifically, proteomic techniques have been used to identify unique oxidation site on creatinase kinase, actin and triosephosphate isomerase in meat sample
and to investigate the relationship between post-mortem sarcoplasmic proteome
and oxidation generation duringstorage and processing, as well as predictive markers that are sensitive to
oxidative stress in meat sample .
The adoption of advanced instrumentation techniques to extract and isolate bioactive
compounds from plant materials, which have been used for phytoremediation,
might provide an alternative solution to the meat industry for overcoming the challenge of oxidative instability in meat.