Observations were made on survival rate in MVI, and stomata size measuring with ocular micrometer in MV the plantlets with stomata about 2 times the normal size were selected for chromosome number counting. The plantlets that possessed the tetraploid chromosome were later selected for further study. 100 plantlets from each treatment of tetraploid plant were multiplied for 4 generations. They were rooted on MS med a without hormone (Silayoi. 1985). The rooted plan hits were then transplanted to pots and to field respectively. In the field the height circumference pseudo stem. Number of leaf and number of sucker were observed.