Although inulin can be converted into fructose by chemical approach, this is associated with some drawbacks. Microbial inulinase yields 95% pure fructose after one step enzymatic hydrolysis of inulin. Thus microbial inulinases are important class of industrial enzymes that have gained much attention in recent times. Inulinases can be produced by many of the microorganisms includ-ing strains of Aspergillus sp., Penicillium sp. and Kluyveromyces sp. Owing to the cost of pure inulin, alternate inulin containing raw, inexpensive substrates are preferred for microbial inulinase production