Cabbages were washed, dried, cut into small pieces and put into
5 L pickle jars along with spices, including garlic (3%), Chinese
prickly ash (1.5%), red peppers (3%) and ginger (2%). In this study,
1000 g cabbages were used for each starter culture fermentation.
Pickle jars filled with the materials were then sterilized at 105 C for
15 min. Cool sterile 2000 ml water containing 6% salt and 3% crystal
sugar was then added into the cool pickle jar. For fermentation
using starter cultures, 0.1% (v/v) of cell suspension (106 cfu/mL) of
each LAB strain was inoculated. The jar was sealed with water to exclude air, and then kept at ambient temperature (20e25 C) for 7
days. Four jars were made and monitored for each LAB strain
(n ¼ 4). During the fermentation, brine was sampled aseptically
every 12 h and divided into two portions. One portionwas used for
analysis of LAB flora and measurements of pH value and the other
portion was stored at 20 C for HPLC analysis.
Cabbages were washed, dried, cut into small pieces and put into
5 L pickle jars along with spices, including garlic (3%), Chinese
prickly ash (1.5%), red peppers (3%) and ginger (2%). In this study,
1000 g cabbages were used for each starter culture fermentation.
Pickle jars filled with the materials were then sterilized at 105 C for
15 min. Cool sterile 2000 ml water containing 6% salt and 3% crystal
sugar was then added into the cool pickle jar. For fermentation
using starter cultures, 0.1% (v/v) of cell suspension (106 cfu/mL) of
each LAB strain was inoculated. The jar was sealed with water to exclude air, and then kept at ambient temperature (20e25 C) for 7
days. Four jars were made and monitored for each LAB strain
(n ¼ 4). During the fermentation, brine was sampled aseptically
every 12 h and divided into two portions. One portionwas used for
analysis of LAB flora and measurements of pH value and the other
portion was stored at 20 C for HPLC analysis.
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