However, T. delbrueckii monoculture had a better ability to retain the odour-active terpenes and terpenoids derived from lychee fruits. The lychee wine fermented with simultaneous culture had a similar characteristic to that of the S. cerevisiae monoculture. In sequential fermentation, prior inoculation of T. delbrueckii played an important role in lowering volatile acids (odour activity value, OAV8 mL/100 mL), higher alcohols (isoamyl alcohol and 2-phenylethyl alcohol) and esters (ethyl esters of octanoate, decanoate and hexanoate, and 2-phenylethyl acetate), relative to the T. delbrueckii monoculture. Therefore, sequential fermentation could accord the wine volatiles from both lychee fruits and biotransformation.