A series of concentrations of methyl parathion solutionswas tested in this manner and the results are presented.As shown in Fig. 5b, in scanning the barcodes, at low concentrationsof methyl parathion (>0.1 μg mL−1) that have highY values (≥60%), the detection region of the barcode appears asa wide bar, resulting in the app reading the assay as the negativeresult “−”. At higher concentrations of methyl parathion(≥0.5 μg mL−1), the Y value became low (<50%); thus the detectionregion appears as a single narrow bar and the app scansas “+”, i.e., a positive result was reported. It is evident that thethreshold value to produce a positive result, which indeedreads as a “+” character, was in between 0.1 and 0.5 μg mL−1.Beyond the qualitative aspect of the barcode assay, we furtherdetermined the yellow intensity of the samples based on thescreen capture image to quantitate the pesticide concentrationin standard or unknown samples.
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