Measurements of soil N2O fluxes were conducted under two conditions i.e., with NO3 or with NO3 plus glucose, respectively. In experiment one, soils were amended with KNO3 (100 mgNg1 soil) together with respective antibiotics. In experiment two, soils were amended with glucose (2 mg C g1 soil) and KNO3 (100 mgNg1soil) together with respective antibiotics. All the chemicals were dissolved in 2 mL distilled water and added dropwise to soil, leading to 80% WFPS. Then, soils were pre-incubated overnight at 4 C in a refrigerator to allow for antibiotics and NO3/glucose diffusion into soil pores. For each experiment, there were a total of 300 microcosms, representing three replicates (i.e., field blocks), five substrate treatments, four antibiotic treatments, and five sampling times.