serves as inoculum and a new fermentation batch starts [8]. Seek-ing for high strength vinegar fermentation is always the mission ofvinegar producers. Generally speaking, high strength vinegar fer-mentation means acetification process with acetification rate andvinegar stoichiometric yield at high level. Many technical devicesand modifications have been tried to optimize vinegar fermenta-tion [9–13]. Among these advances the Frings acetator technologyis the most common application for the commercial productionof all kinds of vinegar. Energy consumption is just 400 W/L ethanolwhich is much lower than aerated-stirred fermentors, and the max-imum vinegar stoichiometric yield is as high as 95%. Commercialsizes of acetators are sufficient for the conversion of up to 3600 Lpure ethanol in 24 h. The Frings alkograph installed in the aceta-tor is an automatic instrument for online measuring the amountof ethanol in the fermenting liquid, which guarantees the smoothrunning of high strength vinegar fermentation [14].In vinegar fermentation, acetification is directly associated withbacterial alcohol oxidation ability which is mainly determined bycellular growth status of AAB. In semi-continuous method, cellulargrowth status could be regulated by discharge/charge operation[15]. The most common operation for high acidity vinegar pro-duction by a few Gluconoacetobacter species is removing 40–50%(v/v) of the working volume [1,14]. However, such volume may notbe suitable for species from the genus Acetobacter. Therefore, theoptimal volume for Acetobacter should be obtained from practical