SEC analysis
The SEC analysis of the water soluble fraction of the
bioemulsifier showed fairly narrow molecular weight
distribution with rather low average molecular weight
(Mw) of ca. 1000 Da obtained using pullulan calibration
standards. Hence, the bioemulsifier should contain
mono-dispersed oligosaccharides. The preliminary qualitative
sugars analysis carried out by acid methanolysis
revealed the presence of glucose as the major constituent
of water-soluble oligosaccharides. Further evidence
for the presence of oligosaccharides in the composition
of the bioemulsifier was obtained using 13C CP-MAS
NMR and 1H NMR analysis.