500 mg of each powered plant material was mixed with 10 ml
of 99.5% ethanol in a 100 ml Erlenmeyer flask and covered with
parafilm. Samples were shaken at 120 rpm for 24 h in the dark
at room temperature, after which the resulting cell organic solvent
extracts were filtered. The organic phase was centrifuged at
1500 rpm for 10 min and filtered under reduced pressure. The wild
plant extract was also partitioned three times with water:diethyl
ether (30 ml each time) and evaporated, since wild plants have a
complex matrix