In the bacteriological investigation of the transmission route,
samples from environmental surfaces of critical and noncritical
areas were collected by wiping with a sterile swab moistened with
sterile normal saline and then inoculated on a 5% sheep’s blood agar
plate (Kyokuto Pharmaceutical Industrial Co, Ltd, Tokyo, Japan).
Bacterial identification and antimicrobial sensitivity tests were
performed with the MicroScan WalkAway 96 Plus System
(Siemens, Erlangen, Germany). To confirm the identification of A
baumannii, OXA-51elike genes were examined by polymerase
chain reaction (PCR).