Microcrystalline chitosan (MCCh) in the form of a gelatinous water dispersion was prepared according to a previously reported
method by which chitosan was regenerated via adding NaOH solution into chitosan solution at constant stirring (Struszczyk, 1987)
The final pH of the dipsersion was 10.0 and the total polymer content amounted to 1% (w/w), including chitosan and gelatin.
AgNPs-chitosan composite was synthesized by adding 2ml of freshly prepared 1.0×10−2 mol/l AgNO3 solution to 50 ml of MCCh gelatinous water dispersion, at constant stirring and room temperature.The reaction was kept for 60 min and the precipitate was filtered, washed and air-dried