However, this method is labor-intensive, time-consuming and has limited sensitivity especiallyfor detecting the disease in the low-prevalent endemic area and inindividuals with low worm burden. In addition, it cannot be usedfor early detection (Lier et al., 2009; Luo et al., 2009; Utzinger et al.,2001; Yu et al., 1998; Zhang et al., 2009; Zhu, 2005).