The TDF (total dietary fiber), a measure of the sum of insoluble and soluble dietary fibers that is based on the digestion of food samples (1 g) with enzymes, was determined as described by Asp et al. Dry sample was suspended in sodium phosphate buffer (pH 6.0) and treated with different enzymes, including termamyl (pH 1.5), pepsin (pH 6.8) and pancreatin (pH 4.5), for 1 h. at specific pH. The enzyme digestate was then filtered using acid-washed celite on a Fibertec system E1023 filtration unit (Tecator, Sweden). Following the filtration of the enzyme digestate, the remaining residue was the IDF and the filtrate was the SDF. The IDF was washed with ethanol and acetone and finally incinerated and weighed. Soluble dietary fiber was estimated by precipitating the filtrate using ethanol. The precipitate was washed with ethanol and acetone, dried, incinerated and finally weighed.