To understand the changes of soilmicrobial activity after biochar application,
the microbial biomass carbon (MBC) contents were determined
at 0 d, 21 d, 63 d, and 105 d of incubation. Results indicate that
the biochar application significantly increased theMBC at the beginning
of incubation, 63 d and 105 d (only in 5% application rate). The differences
were statistically significant (p b 0.05), except for the analytical
results at 21 d (Fig. 3). In addition, the highest contents of MBC were
found at 21 d for each treated soil, which were 3200 mg kg−1 for 5%
biochar-amended soil, 1145 mg kg−1 for 2.5% biochar-amended soil
and 1759 mg kg−1 for the control, respectively.