The emulsifying capacity of whey protein in b-LGI and
WPF was studied in emulsions containing 4 and 18% fat at
varying concentrations of whey protein. Before recombination,
the whey protein content of b-LGI and WPF was determined
using the Kjeldahl technique. Then, WPF was
concentrated to increase the whey protein concentration to
different values in the 0.15–1.50% range and solutions of
b-LGI were prepared at whey protein concentrations varying
from 0.15 to 0.75%. The efficiency of each WPF
concentrate and each solution of b-LGI in emulsifying anhydrous milk fat at 40–508C was determined by particle
size measurements immediately after processing.
The weight average particle size (d4,3) is shown in
Fig. 3. As fat content did not affect initial particle
size at similar protein-to-fat ratios, particle size was
plotted against protein-to-fat ratio. Increasing the
protein-to-fat ratio led to a rapid decline in d4,3, irrespective
of the whey protein material used in the
system. Thus, whey proteins in WPF and b-LGI were
very effective in covering fat surfaces and consequently, in
inhibiting immediate coalescence. Similar results were
observed by Klemaszewski et al. (1992) for emulsions
(40% peanut oil-in-water) prepared with pure whey protein
material (b-lg, a-la and BSA) and by Agboola and Dalgleish
(1995) for emulsions (20% soya oil-in-water) prepared
with b-lg.
The emulsifying capacity of whey protein in b-LGI andWPF was studied in emulsions containing 4 and 18% fat atvarying concentrations of whey protein. Before recombination,the whey protein content of b-LGI and WPF was determinedusing the Kjeldahl technique. Then, WPF wasconcentrated to increase the whey protein concentration todifferent values in the 0.15–1.50% range and solutions ofb-LGI were prepared at whey protein concentrations varyingfrom 0.15 to 0.75%. The efficiency of each WPFconcentrate and each solution of b-LGI in emulsifying anhydrous milk fat at 40–508C was determined by particlesize measurements immediately after processing.The weight average particle size (d4,3) is shown inFig. 3. As fat content did not affect initial particlesize at similar protein-to-fat ratios, particle size wasplotted against protein-to-fat ratio. Increasing theprotein-to-fat ratio led to a rapid decline in d4,3, irrespectiveof the whey protein material used in thesystem. Thus, whey proteins in WPF and b-LGI werevery effective in covering fat surfaces and consequently, ininhibiting immediate coalescence. Similar results wereobserved by Klemaszewski et al. (1992) for emulsions(40% peanut oil-in-water) prepared with pure whey proteinmaterial (b-lg, a-la and BSA) and by Agboola and Dalgleish(1995) for emulsions (20% soya oil-in-water) preparedwith b-lg.
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