2.1.2. Enzyme
Commercial soluble lipase from T. lanuginosus was purchased
from Novo Nordisk (Denmark). It is produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism. The enzyme activity was 16.3 U/ml. One unit of activity (U) is
defined as the amount of enzyme forming 1 lmol p-nitrophenol
per minute from p-nitrophenyl palmitate using lipase assay
method. Protein determinations were carried out using Bradford
method (Bradford, 1976).