There is a difference between halophilic bacteria and halotolerant bacteria. Indeed, the term "halophilic"
means that microorganisms require the presence of salt (NaCl) in the medium for their growth. However,
the term "halotolerant" means that microorganisms tolerate different salt concentrations but not required
during growth (Table.1). (Kushner D. J., 1985)
To assess the influence of salt stress on the biodegradation of carbon and nitrogen composed and on the
tolerance of microorganisms to adapt to this specific environment (the halotolerant bacteria), the
respirometer were inoculated with fresh activated sludge obtained from a local sewage treatment plant
'IBN ZIAD' Constantine, Algeria designed to treat 800l/s (6916 m3/day) flow rate or 450000 population
equivalent/d.
Sampling was carried out with a bottle of immersion (Fig .2); the bottle allows us to access multiple
levels and allowing the sample to each desired level. The collected sludge was used immediately and no
adjustment procedure was undertaken.