Statistical analysis
Data for various algal residues were analyzed using the
Proc Glimmix of SAS version 9.2 [14]. Differences between
least square means were tested using Satterthwaite
approximation for the denominator degrees of freedom as
an option. Quadruplicate laboratory analyses were conducted
for in vitro gas production analysis. Four runs of
gas measurements within variation less than ± 5% at
72 h incubation were used for the analysis. Data fitting a
non-linear model were analyzed using Proc NLIN of
SAS [14], which was developed by Dr. P. J. Weimer
(personal communication).