These results seem to confirm a relationship between stress tolerance and glycerol overproduction, even though this relationship has been mostly studied in relation with osmotic stress. Stanley, Fraser, et al. (2010) also found ethanol tolerant strains to overproduce glycerol and acetic acid; the later only in the presence of ethanol, but this would be the
case after some hours of fermentation during winemaking. McBryde et al. (2006) evolved a wine strain not able to finish
fermentation on a 20% sugars synthetic must and producing high levels of acetic acid (1.7 g/L), and the evolved mixed culture also overproduced glycerol (112%) and slightly more acetic acid (106%). A sensory analysis was carried out on wines made with lower sugar addition by a four-taster panel, at bottling and 6 months after bottling. Wine made with L76M2 was the preferred one. Wines made with the higher sugar content were not tasted due to differences found in final sugar concentration that would bias the result.