Plant samples except for one part of potato tuber, which was
brought back from field, were dessicated at 105 ◦C for 30 min and
oven-dried at 70 ◦C in a forced air circulation oven until a constant
weight was reached (Silva et al., 2013) and ground to pass a 2mm
sieve (Zebarth et al., 2012). The total N concentration of plant was
determined by H2SO4- H2O2 digestion and the micro-Kjeldahl procedure
(Douglas et al., 1980). N accumulation (NA) in plants was
calculated from the sum of the dry matter and N concentration of
the different plant parts. Total apparent N use efficiency (NUE), N
agronomic efficiency (NAE) and N physiological efficiency (NPE)
were calculated by the following formulas
Plant samples except for one part of potato tuber, which wasbrought back from field, were dessicated at 105 ◦C for 30 min andoven-dried at 70 ◦C in a forced air circulation oven until a constantweight was reached (Silva et al., 2013) and ground to pass a 2mmsieve (Zebarth et al., 2012). The total N concentration of plant wasdetermined by H2SO4- H2O2 digestion and the micro-Kjeldahl procedure(Douglas et al., 1980). N accumulation (NA) in plants wascalculated from the sum of the dry matter and N concentration ofthe different plant parts. Total apparent N use efficiency (NUE), Nagronomic efficiency (NAE) and N physiological efficiency (NPE)were calculated by the following formulas
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