Also, the effect of pH on pectinase production was studied at a pH range of 6.0- 10. The optimum pH for pectinase production by B.licheniformis was 9.0 with enzyme activity of 0.1505μmol/ml/min. Most Bacillus species have been reported to produce pectinase maximally at alkaline pH ranging from 7.0 to 9.0