The fermented wines exhibited very high contents (above 17 g/
L) in glycerol as response of yeast to the osmotic stress caused by
the high concentration of sugars present. The molecular response
of S. cerevisiae to a high glucose concentration in must is very
complex and influenced by a number of factors. Under these conditions,
S. cerevisiae accumulates glycerol, which is the main
compatible metabolite (García-Mauricio & García-Martínez, 2013).
The production of glycerol is countered by that of other metabolites
such as acetic acid, acetaldehyde, acetoin and 2,3-butanediol
(Moreno & Peinado, 2012), which is intended to balance the
redox potential. All these compounds exhibited increased concentrations
in the fermentation batches involving reused immobilized
yeast cells; these carbonyl compounds are typical by-products of
glyceropyruvic fermentation, which is high active at the beginning
of the of the fermentation and under the hyperosmotic conditions
(Moreno & Peinado, 2012).
The fermented wines exhibited very high contents (above 17 g/
L) in glycerol as response of yeast to the osmotic stress caused by
the high concentration of sugars present. The molecular response
of S. cerevisiae to a high glucose concentration in must is very
complex and influenced by a number of factors. Under these conditions,
S. cerevisiae accumulates glycerol, which is the main
compatible metabolite (García-Mauricio & García-Martínez, 2013).
The production of glycerol is countered by that of other metabolites
such as acetic acid, acetaldehyde, acetoin and 2,3-butanediol
(Moreno & Peinado, 2012), which is intended to balance the
redox potential. All these compounds exhibited increased concentrations
in the fermentation batches involving reused immobilized
yeast cells; these carbonyl compounds are typical by-products of
glyceropyruvic fermentation, which is high active at the beginning
of the of the fermentation and under the hyperosmotic conditions
(Moreno & Peinado, 2012).
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