Biodiesel wastewater was used as feed in this study. It was collected from UTHM Biodiesel Pilot Plant which is
situated in University Tun Hussein Onn Malaysia. This Plant uses palm oil as a feedstock and alkali-catalyzed
transesterification process. The sample then kept in a cold room at temperature of 4°C to avoid further
biodegradation. The characteristic of this biodiesel wastewater were analyzed according to the standard methods for
examination of water and wastewaters (APHA, AWWA & WEF, 2012) are shown in Table 1.