2.2. Toxicity assay using brine shrimp (A. salina)
Eggs (25 mg) from A. salina were acquired from local pet shops
and hatched at 25–30 C in natural seawater (pH 8.0). After 24 h,
the newly hatched larvae were collected and used in the lethality
assay according to procedures described by Meyer et al. (1982).
Groups of 12–15 larvae were exposed to MvRL (5 mL, 10–300 mg/
mL) diluted in natural seawater and, after 24 h, the survival rates
(%) were recorded. In the control group, larvae were incubated in
seawater. Three independent experiments were performed in
triplicate.