Cheese samples and preparation of cheese-based broth
The effects of TVEO on bacterial cell viability were examined
using a coalho cheese-based broth and a semi-solid coalho cheesemodel to simulate the environmental conditions of the microorganisms in the studied food matrix. The coalho cheese samples (two
units of 500 g from the same batch, produced using only enzymatic coagulation with 0.9 mL/L chymosin, without the addition of mesophilic lactic acid culture) used to prepare the cheese-broth and assays using the semi-solid cheese model were purchased from a local retail supermarket in Jo~ao Pessoa (Brazil).