Fed-batch fermentation strategies were studied in order to fur-ther explore the potential of GALG20. Exponential feeding waschosen and solutions with different concentrations of glucose weretested in order to determine the best feeding profile in termsof pDNA productivity and cost–benefits. Plasmid amplificationwas induced at high temperature (42◦C) since the model vector,pVAX1GFP, is a pUC-based vector (Lin-Chao et al., 1992). Glucose