Various genes related to formation of the appressorium (a specialized structure involved in penetration of the insect cuticle by the fungus), virulence, and nutritional stress had been cloned from M.anisopliae.Additional copies of the PrI gene,which encodes a subtilisin-like protease involved in host cuticle penetration were engineered into the genome of M. anisopliae. The larvae infected with recombinant strains died 25% sooner and feeding damage was reduced by 40% (St. Legeret al., 1996). The prospect of using recombinant fungi for insect control highlights the need for further research in identifying and manipulating genes involved in pathogenesis and monitoring of genetic exchange between strains by using isolate-specific molecular markers. (Harrison and Bonning, 1998). Despite a potentially wide array of insecticidal proteins produced by entomopathogenic fungi, fungal genes have played little part in agricultural biotechnology to date.