1 mL of the resulting slurry was transferred
into a test tube containing 2 mL of trichloroacetic acid/thiobarbituric
acid (TCA/TBA) solution consisting of 15% TCA (w/v) and 0.375%
TBA (w/v) in 0.25 M HCl and 3 ml 2% butylated hydroxytoluene (w/v)
prepared in absolute ethanol in a test tube and, the tube content was
immediately vortexed. Following water bath treatment at 100 °C for
15min, the tube contentwas cooled rapidly down to roomtemperature
and centrifuged at 1000 ×g for 10min.