Lactic Acid Bacteria (LAB) were isolated from cereals and seeds from Argentinean markets. Colonies
representing different morphological appearances were isolated and differentiated based on phenotypic
characteristics. Fifty strains were identified by 16S rRNA gene sequencing analysis obtaining LAB from
genera Enterococcus, Lactobacillus and Pediococcus. Technological and nutritional characteristics (acidifying
capacity, antimicrobial production, proteolytic activity, folate production) were analyzed. Lactobacillus
pentosus ES124 and Lactobacillus plantarum ES137 presented high production of folate (61 and
57 ng/mL, respectively) as did Enterococcus mundtii ES63 which reached a total folate production of
70 ng/mL. Six LAB strains produced bacteriocin-like inhibitory substances (BLIS) with antilisterial activity.
Eight strains were selected for use as sourdough starters and in situ applications based on their
important technological properties.