The attached particles were collected one by one with the tweezers.
They were dissolved in the heated water (40 ◦C) and the suspended solid were removed by filtration, then the filtrate was freeze-dried.
After measurement of weight of all the freeze-dried products,
it was dissolved in distilled water at 100 mg/L
and the cordycepin concentration was determined by the HPLC.
The cordycepin content in the crystallized compounds was calculated based on the cordycepin concentration of the said solution