Fungal species causing fruit rot of jackfruit have been isolated from seven different locations of Birbhum
and Burdwan districts of West Bengal, India. Each isolate showed more or less similar microscopic characteristics.
A representative strain VBAM1, isolated from a severely infected jackfruit was identified as
Rhizopus stolonifer by 28S rDNA sequence homology. Increased reducing sugar content in pectin broth
indicates pectinase production by the pathogen. The pathogen was not inhibited by P500 lg/ml of
Mancozeb and Bavistin. Copper oxychloride, Blytone 50% a.i. showed antifungal activity at comparatively
lower concentration (200 lg/ml). Two rhizospheric bacterial strains, Burkholderia cenocepacia VBC7 and
Pseudomonas poae VBK1, and three different strains of Lactococcus lactis subsp. lactis can produce significant
zones of inhibition against the pathogen in dual culture overlay plates. They induced mycelia breakage
of pathogen as evidenced from scanning electron micrographs. When applied to whole plants, the
strains reduced or prevented disease and when applied postharvest to Rhizopus inoculated fruit delayed
and/reduced disease incidence. These agents were also re-isolated from the applied surfaces and survived
for long time when mixed with suitable carrier base indicating stability in a formulation over time
Fungal species causing fruit rot of jackfruit have been isolated from seven different locations of Birbhumand Burdwan districts of West Bengal, India. Each isolate showed more or less similar microscopic characteristics.A representative strain VBAM1, isolated from a severely infected jackfruit was identified asRhizopus stolonifer by 28S rDNA sequence homology. Increased reducing sugar content in pectin brothindicates pectinase production by the pathogen. The pathogen was not inhibited by P500 lg/ml ofMancozeb and Bavistin. Copper oxychloride, Blytone 50% a.i. showed antifungal activity at comparativelylower concentration (200 lg/ml). Two rhizospheric bacterial strains, Burkholderia cenocepacia VBC7 andPseudomonas poae VBK1, and three different strains of Lactococcus lactis subsp. lactis can produce significantzones of inhibition against the pathogen in dual culture overlay plates. They induced mycelia breakageof pathogen as evidenced from scanning electron micrographs. When applied to whole plants, thestrains reduced or prevented disease and when applied postharvest to Rhizopus inoculated fruit delayedand/reduced disease incidence. These agents were also re-isolated from the applied surfaces and survivedfor long time when mixed with suitable carrier base indicating stability in a formulation over time
การแปล กรุณารอสักครู่..
