The solvent extraction procedure was carried out
according to the extraction procedures described by
Khoo (2009) with slight modifications. A required
amount of pegaga (Centella asiatica) dry powder
according to experimental design was weighed
accurately using analytical balance (AB204-S,
Mettler Toledo, Switzerland) and mixed with 60 mL
of ethanol in a conical flask which was wrapped with
parafilm (Pechiney plastic packaging, USA) and
aluminium foil (Diamond, USA) to prevent spilling
of mixture and light exposure, respectively. The
mixture was then shaken at rotation level 8 using
a temperature controlled water bath shaker (WNB
7-45, Memmert, Germany) for 100 min at 56.8°C.
After extraction, the pegaga (C. asiatica) extract
underwent filtration using Whatman No. 1 filter
paper (Whatman International, England) to obtain
a clear solution of crude extract. The crude extract
was collected in a light-protected amber bottle for
analysis without storage. All extractions were carried
out in replicate.
The solvent extraction procedure was carried outaccording to the extraction procedures described byKhoo (2009) with slight modifications. A requiredamount of pegaga (Centella asiatica) dry powderaccording to experimental design was weighedaccurately using analytical balance (AB204-S,Mettler Toledo, Switzerland) and mixed with 60 mLof ethanol in a conical flask which was wrapped withparafilm (Pechiney plastic packaging, USA) andaluminium foil (Diamond, USA) to prevent spillingof mixture and light exposure, respectively. Themixture was then shaken at rotation level 8 usinga temperature controlled water bath shaker (WNB7-45, Memmert, Germany) for 100 min at 56.8°C.After extraction, the pegaga (C. asiatica) extractunderwent filtration using Whatman No. 1 filterpaper (Whatman International, England) to obtaina clear solution of crude extract. The crude extractwas collected in a light-protected amber bottle foranalysis without storage. All extractions were carriedout in replicate.
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