Mice were randomly divided into six groups of five animals per group.
Graded doses of the extract (400, 800, 1600, 3200 and 6400 mg/kg) were administered to the animals orally ad libitum.
The control group was administered 0.1 ml distilled water orally.
Mice were observed for 24 h post-treatment for mortality, behavioural changes (restlessness, dullness, agitation) and signs
of toxicity.