Testing of filter paperase activity can reflect the general cellulase activity because the substrate used for testing was Whatman filter paper no. 1 (crystal fibers properties) that involves the activity of C1 which acts as an activator of crystalline cellulose into reactive cellulose [17]. Based on the analysis of variance, it showed that the interaction of treatment and the concentration of bagasse fermentation time was highly significant (p < 0.01) on cellulase activity.