For the GLC study,
methyl ester of oil was prepared and fatty acid compositions of the
methyl esters were analysed by Hawlett Packard instrument (model
26890) supplied with split inlet, flame ionisation detector (FID). A
DB 225 column (30 m x 0.25 mm ID, 0.25 μm film) was used. The
carrier gas was Nitrogen 1 ml/min column flow and 1: 50 split ratio.
Injector and detector temperatures were 230 and 260°C,
respectively. Oven temperature was initially set at 90°C for 5 min
and then gradually increased to 130°C by the rate of 3°C/min; keptat 130°C for next 12 min. and than temperature was raised up to
230°C at rate of 2°C/min.