2 mL THF dissolving -C was added dropwise into the polymer solution to give the final feed con-centration of -C ranging from 0.05 to 2.0 mg/mL while beingsonicated for 30 min by a probe-based sonicator. Then, the organicsolvent was removed from the mixture solution at 37◦C by rotaryevaporation. The solution was consequently adjusted to CS-g-PLAconcentration of 1 mg/mL and filtered through a 0.45 m mem-brane filter to remove free -C. The resultant solution was storedat 4◦C for subsequent analysis.