TEMPO® system
Aliquots containing different levels of inoculum were transferred to TEMPO® EB flasks (Biomérieux S.A.). These aliquots were diluted and automatically transferred to a card containing 48 wells of three different volumes (16 x 225, 16 x 22.5, 16 x 2.25 µL), using TEMPO Filler®. After incubation for 24 hours at 35 °C, the results were read on the TEMPO Reader®.