Three different tests (MIC, DD and FB) were applied to evaluate the antimicrobial activities of the metabolites against ginseng pathogens. On average, percent inhibition recorded by the MIC test was higher than the values reported by the DD and FB test values (Tables 1 and 2). For example, the percent inhibition of the P. terrestris extract against R. solani was 96.8%, 52.2% and 72.1% in the MIC, DD and FB test, respectively. These results indicate that the inhibition of pathogen growth depends on the treatment method. In the MIC test, the extract directly contacted pathogens through filter paper, while it indirectly contacted pathogens in the DD test, and directly contacted ginseng pathogens on the agar plate during the FB test. The FB test has potential for commercial applications because it does not require metabolite extraction. However, it was not possible to use the same concentration of fermentation broth, which is important for commercialization. P. terrestris extract showed the best antimicrobial activity against all five ginseng pathogens, although the extracts from five endophytes also showed good antimicrobial activities against at least one of the ginseng pathogens