Statistical Analysis
All statistical analyses were performed with SPSS (Version 18.0).
Experiment 1—Six drug-treated animals had cannulae misplaced outside the MPOA
(Figure 1); a Student's t test was used to assess any behavioral differences between these
animals and vehicle-treated exposed controls. This analysis revealed no significant
differences in copulatory behavior between these two groups. Therefore, subsequent
analyses reported below combined drug-treated animals with misplaced cannulae together
with vehicle-treated exposed controls; this grouping is referred to as “combined controls
with exposure.” Thus, the final groups used for analyses were as follows: combined controls
with exposure (n=16), 1μg SCH-23390 with exposure (n=13), 3μg SCH-23390 with
exposure (n=10), and vehicle-treated unexposed controls (n=9).
One-way between-subjects ANOVAs were used to assess differences between groups for all
copulatory and motoric activity measures described above, and post-hoc analyses (Tukey's
Honestly Significant Difference, HSD) were subsequently performed to reveal differences
between specific groups. In cases where fewer than 3 subjects per group performed a
behavior, that group was excluded from statistical analysis. For all statistical analyses, p <
0.05 was considered statistically significant and p < 0.10 was considered a trend.
Experiment 2—One-way analyses of variance (ANOVA) were used in conjunction with
Tukey's HSD post-hoc analyses to probe for differences in relative band densities between
groups