For
example, PCR is mainly used for sexing embryos and
can be nearly 100% accurate, but involves many inconvenient
and time-consuming steps. In addition, PCR
utilizes complicated equipment, such as thermal cycling
machines and/or electrophoresis apparatus, which
are not always available in the field. It was reported that
the addition of Ampl-Y into reaction tubes under
ultraviolet transilluminator could replace electrophoresis
electrophoresis
for PCR-based sexing embryo and shorten detection
time