The biofilm formation by V. fischeri depends on the presence
of a polysaccharide gene cluster, syp, and a number of
syp regulators, including the histidine sensor kinase RscS.
By over-expressing the RscS sensor kinase, Shibata and
Visick induced OMVs production in a manner that depends
on the presence of the syp locus. These OMVs contained
antigenically distinct molecules, possibly representing
modified forms of lipopolysaccharide (LPS). Their findings
support the correlation between OMVs production and biofilm
formation