These clones were selected for plasmid DNA isolation.
The same size fragments corresponding to the RAPD markers were
recovered from Hind III and Eco RI double digested plasmid DNA. The
recombinants were sequenced. The first ten nucleotides at both ends
of the sequences obtained matched completely with their corresponding
RAPD primers OPA 01 and OPE 18 (Fig. 3). For both the RAPD
markers ‘Cur 01’ and ‘Cur 02’, the sequence alignment data revealed
100% similarity between sequences obtained from C. zedoaria, C.
malabarica and the market sample II. Nucleotide sequence of ‘Cur 01’
consisted of 593 bp with 40.30% G+C content (A=174; C=151;
G=88; T=180). The length of the ‘Cur 02’ obtained was 377 bp with
36.87% G+C content (A=111; C=62; G=77; T=127). Blast results
revealed that both the DNA sequences shared only partial homology
with known plant nucleotide sequence.