The protein dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex (FTT_0077) was immunoreactive with 11 of the 12 patient sera screened but none of the control sera.
Due to limited volumes or sera, a single serum sample from patient 1671 was screened against antigen separated in the pH range 6–11 (Fig. 2a).
Some background interference was observed in the low MW region of the blot, and a single area of immunoreactivity was observed in the basic high molecular weight region.
This protein was not able to be identified and is indicated by arrows in Fig. 2e.